Journal
ONCOGENE
Volume 42, Issue 8, Pages 613-624Publisher
SPRINGERNATURE
DOI: 10.1038/s41388-022-02579-1
Keywords
-
Ask authors/readers for more resources
The roles of PCNA as a scaffold protein in DNA replication and repair are well known, but its functions in the cytosol are less understood. ENO1 and 6PGD, two metabolic enzymes, interact with PCNA. Mutation of the PCNA interacting motif APIM in ENO1 resulted in reduced ENO1 protein levels, impaired growth rate, decreased glucose consumption, and reduced AKT activation. The interaction between PCNA and ENO1 has significant effects on metabolism.
The essential roles of proliferating cell nuclear antigen (PCNA) as a scaffold protein in DNA replication and repair are well established, while its cytosolic roles are less explored. Two metabolic enzymes, alpha-enolase (ENO1) and 6-phosphogluconate dehydrogenase (6PGD), both contain PCNA interacting motifs. Mutation of the PCNA interacting motif APIM in ENO1 (F423A) impaired its binding to PCNA and resulted in reduced cellular levels of ENO1 protein, reduced growth rate, reduced glucose consumption, and reduced activation of AKT. Metabolome and signalome analysis reveal large consequences of impairing the direct interaction between PCNA and ENO1. Metabolites above ENO1 in glycolysis accumulated while lower glycolytic and TCA cycle metabolite pools decreased in the APIM-mutated cells; however, their overall energetic status were similar to parental cells. Treating haematological cancer cells or activated primary monocytes with a PCNA targeting peptide drug containing APIM (ATX-101) also lead to a metabolic shift characterized by reduced glycolytic rate. In addition, we show that ATX-101 treatments reduced the ENO1 - PCNA interaction, the ENO1, GAPDH and 6PGD protein levels, as well as the 6PGD activity. Here we report for the first time that PCNA acts as a scaffold for metabolic enzymes, and thereby act as a direct regulator of primary metabolism.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available