The consequence of leaf life span to virus infection of herbivorous insects

Many herbivorous insects die of pathogen infections, though the role of plant traits in promoting the persistence of these pathogens as an indirect interaction is poorly understood. We tested whether winter leaf retention of bush lupines (Lupinus arboreus) promotes the persistence of a nucleopolyhedroviruses, thereby increasing the infection risk of caterpillars (Arctia virginalis) feeding on the foliage during spring. We also investigated whether winter leaf retention reduces viral exposure of younger caterpillars that live on the ground, as leaf retention prevents contaminated leaves from reaching the ground. We surveyed winter leaf retention of 248 lupine bush canopies across twelve sites and examined how it related to caterpillar infection risk, herbivory, and inflorescence density. We also manipulated the amount of lupine litter available to young caterpillars in a feeding experiment to emulate litterfall exposure in the field. Greater retention of contaminated leaves from the previous season increased infection rates of caterpillars in early spring. Higher infection rates reduced herbivory and increased plant inflorescence density by summer. Young caterpillars exposed to less litterfall were more likely to starve to death but less likely to die from infection, further suggesting foliage mediated exposure to viruses. We speculate that longer leaf life span may be an unrecognized trait that indirectly mediates top-down control of herbivores by facilitating epizootics.

Automated summary

Winter leaf retention promotes the persistence of nucleopolyhedroviruses on bush lupines, increasing the infection risk of caterpillars feeding on the foliage. Leaf retention also reduces viral exposure for younger caterpillars on the ground. Higher infection rates in early spring due to greater retention of contaminated leaves lead to reduced herbivory and increased plant inflorescence density by summer. Young caterpillars exposed to less litterfall are more likely to starve but less likely to die from infection, suggesting foliage-mediated exposure to viruses.