Hexagonal cobalt oxide nanosheet-based enzymeless electrochemical uric acid sensor with improved sensitivity

Enzymeless sensors have been known as highly stable, fast, and reliable devices for the detection of several biomolecules and for use in early disease diagnosis. Hence, it is crucial to fabricate enzymeless sensors with high sensitivity. Herein, we describe the synthesis of hexagonal cobalt oxide nanosheets using a hydrothermal method. The crystalline phase of the as-synthesised cobalt oxide nanosheets was examined using X-ray diffraction (XRD), and the morphology was observed using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The cobalt oxide nanomaterials were synthesised in a large quantity and showed a uniform and hexagonal nanosheet like morphology. To investigate their application, the nanosheet nanomaterial was utilised to fabricate an ultra-sensitive enzymeless electrochemical-based uric acid (UA) sensor on a screen-printed carbon electrode (SPCE). A paste of hexagonal cobalt oxide nanosheets in a conductive binder was drop-casted onto the working electrode of the SPCE. The cobalt oxide nanosheet-based UA sensor showed a good response for UA detection. Under optimal conditions, the cobalt oxide/SPCE UA sensor showed linearity up to 200 mu M of UA with ultra-sensitivity (1560 mu A mM(-1) cm(-2)) and a limit of detection of similar to 5 mu M. Furthermore, the fabricated UA sensor was subjected to reproducibility, storage stability, and selectivity tests, which confirmed good stability, reproducibility, and specificity for UA. The developed ultra-sensitive enzymeless UA sensor is expected to be useful for the sensing of UA in real samples.

Automated summary

Enzymeless sensors are stable, fast, and reliable devices for biomolecule detection and disease diagnosis. In this study, hexagonal cobalt oxide nanosheets were synthesized using a hydrothermal method, and then utilized to fabricate an ultra-sensitive enzymeless electrochemical-based uric acid (UA) sensor. The cobalt oxide nanosheet-based UA sensor showed a good response for UA detection, with ultra-sensitivity and a low limit of detection. The developed sensor demonstrated good stability, reproducibility, and specificity for UA.