Juvenile social isolation affects the structure of the tanycyte-vascular interface in the hypophyseal portal system of the adult mice

Accumulating evidence indicates that social stress in the juvenile period affects hypothalamic-pituitary-adrenal (HPA) axis activity in adulthood. The biological mechanisms underlying this phenomenon remain unclear. We aimed to elucidate them by comparing adult mice that had experienced social isolation from postnatal day 21-35 (juvenile social isolation (JSI) group) with those reared normally (control group). JSI group mice showed an attenuated HPA response to acute swim stress, while the control group had a normal response to this stress. Activity levels of the paraventricular nucleus in both groups were comparable, as shown by c-Fos immunoreactivities and mRNA expression of c-Fos, Corticotropin-releasing factor (CRF), Glucocorticoid receptor, and Mineralocorticoid receptor. We found greater vascular coverage by tanycytic endfeet in the median eminence of the JSI group mice than in that of the control group mice under basal condition and after acute swim stress. Moreover, CRF content after acute swim stress was greater in the median eminence of the JSI group mice than in that of the control group mice. The attenuated HPA response to acute swim stress was specific to JSI group mice, but not to control group mice. Although a direct link awaits further experiments, tanycyte morphological changes in the median eminence could be related to the HPA response.

Automated summary

Accumulating evidence has shown that social stress during the juvenile period affects the activity of the hypothalamic-pituitary-adrenal (HPA) axis in adulthood. In this study, adult mice that experienced social isolation during postnatal day 21-35 (juvenile social isolation (JSI) group) were compared to normally reared mice (control group) to investigate the mechanisms underlying this phenomenon. The JSI group mice showed a weakened HPA response to acute swim stress, and there were morphological changes in the tanycytic endfeet in the median eminence, potentially related to the HPA response.