Journal
NATURE NEUROSCIENCE
Volume 26, Issue 2, Pages 350-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41593-022-01219-x
Keywords
-
Categories
Ask authors/readers for more resources
Identification of structural connections between neurons is essential for understanding brain function. In this study, the researchers developed a systematic mapping process to determine brain-wide monosynaptic input connections in genetically defined neuronal populations and discovered differences in target specificity, layer specificity, and cell class specificity in the visual cortex.
Identification of structural connections between neurons is a prerequisite to understanding brain function. Here we developed a pipeline to systematically map brain-wide monosynaptic input connections to genetically defined neuronal populations using an optimized rabies tracing system. We used mouse visual cortex as the exemplar system and revealed quantitative target-specific, layer-specific and cell-class-specific differences in its presynaptic connectomes. The retrograde connectivity indicates the presence of ventral and dorsal visual streams and further reveals topographically organized and continuously varying subnetworks mediated by different higher visual areas. The visual cortex hierarchy can be derived from intracortical feedforward and feedback pathways mediated by upper-layer and lower-layer input neurons. We also identify a new role for layer 6 neurons in mediating reciprocal interhemispheric connections. This study expands our knowledge of the visual system connectomes and demonstrates that the pipeline can be scaled up to dissect connectivity of different cell populations across the mouse brain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available