Achyranbidens A-C: three new compounds from Achyranthes bidentata Blume

Phytochemical study on the roots of Achyranthes bidentata Blume led to the isolation of sixteen compounds including three new ones (1-3). Their chemical structures were determined as oleanolic acid 28-O-beta-D-glucopyranoside-3-O-[beta-D-glucopyranosyl-(1 -> 3)-beta-D-galactopyranoside) (1), methyl (8Z,11Z)-5,6,7-trihydroxytetradeca-8,11-dienoate (2), methyl (6E,11Z)-5,8,9-trihydroxytetradeca-6,11-dienoate (3), fulgidic acid (4), (9E,11E)-13-oxooctadeca-9,11-dienoic acid (5), (9Z,11E,15Z)-13-hydroxyoctadeca-9,11,15-trienoic acid (6), oleanolic acid 28-O-beta-D-glucopyranoside-3-O-alpha-L-rhamnopyranosyl-(1 -> 4)-beta-D-glucuronopyranoside (7), oleanolic acid 28-O-beta-D-glucopyranoside-3-O-beta-D-glucopyranosyl-(1 -> 2)-[alpha-L-rhamnopyranosyl-(1 -> 3)]-beta-D-glucuronopyranoside (8), oleanolic acid 3-O-beta-D-glucopyranosyl-(1 -> 2)-[alpha-L-rhamnopyranosyl-(1 -> 3)]-beta-D-glucuronopyranoside (9), oleanolic acid 3-O-alpha-L-rhamnopyranosyl-(1 -> 3)-beta-D-glucuronopyranoside (10), blumenol C glucoside (11), citroside A (12), 6S,9S-roseoside (13), ginsenoside Rg1 (14), 20-hydroxyecdysone (15), and benzyl alpha-L-rhamnopyranosyl-(1 -> 6)]-beta-D-glucopyranoside (16) by spectroscopic analysis. Compounds 1, 7 and 11-16 inhibited NO production in LPS-activated RAW264.7 cells with IC50 values in the range from 28.03 to 54.23 mu M (positive control, L-NMMA: IC50 = 35.52 mu M). Compounds 14 and 15 showed anti alpha-glucosidase activity with IC50 values of 176.24 and 156.92 mu M, respectively, compared with the positive control, acarbose, IC50 = 160.99 mu M.

Automated summary

A phytochemical study on the roots of Achyranthes bidentata Blume resulted in the discovery of 16 compounds, including 3 new ones (1-3). Their chemical structures were determined through spectroscopic analysis. Compounds 1, 7, and 11-16 exhibited inhibition of NO production in LPS-activated RAW264.7 cells, with IC50 values ranging from 28.03 to 54.23 μM. Compounds 14 and 15 showed anti-α-glucosidase activity, with IC50 values of 176.24 μM and 156.92 μM, respectively.