Journal
NATURAL PRODUCT RESEARCH
Volume 37, Issue 22, Pages 3884-3888Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2022.2155821
Keywords
Aconitum carmichaeli; antioxidant activity; anti-hepatoma activity; biological activity; HRMS
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Five extracts of Aconitum carmichaeli were obtained from the aerial parts using different extraction methods and purification techniques. The 80%EE extract showed the highest anti-hepatoma activity and the 10%EE and 80%EE extracts exhibited the strongest antioxidant activity. These findings highlight the medicinal potential of the aerial part of Aconitum carmichaeli and its potential as a natural medicine.
Five extracts of the aerial parts of Aconitum carmichaeli were obtained by different solvent extraction or macroporous adsorption resin purification: ethyl acetate layer extract (EAE), n-butanol layer extract (BuE), water layer extract (WE), extract eluted by 10% ethanol from macroporous resin (10%EE), extract eluted by 80% ethanol from macroporous resin (80%EE). Antioxidant activities of the five extracts were determined by ABTS, DPPH, FRAP assays, anti-AChE activities by modified Ellman's method, in vitro anti-hepatoma activities by CCK-8 assay, and chemical constituents of 80%EE were identified by UPLC-QE-Orbitrap-MS. The results demonstrated that the 80%EE showed the best in vitro anti-hepatoma activity on Huh-7 cell line with an IC50 of 103.91 +/- 11.02 mu g/mL. 10%EE and 80%EE gave the highest antioxidant activity. Furthermore, current findings demonstrated that the aerial part of Aconitum carmichaeli Debx. has high medicinal value and may be a good natural medicine.
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