Antimycic Acid and its Acetyl Derivative from Deep-sea-derived Alcanivorax sp. SHA4 with Neuroprotective Properties

Chemical investigation of secondary metabolites of the deep-sea-derived Alcanivorax sp. SHA4 identified a new compound 1 which was antimycic acid (2)'s acetyl derivative, and 11 known compounds (2-12). Their structures were elucidated by extensive nuclear magnetic resonance and mass spectrometry spectroscopic analyses, and the absolute configuration of compound 1 was determined by Marfey's method. Bioactivity assays indicated that compounds 1 and 2 exhibited significant neuroprotective properties against glutamate-induced PC12 cell death in 0.02-0.31 mu M.

Automated summary

Chemical investigation of secondary metabolites from the deep-sea-derived Alcanivorax sp. SHA4 resulted in the identification of a new compound 1, which is the acetyl derivative of antimycic acid (2), along with 11 known compounds (2-12). The structures of these compounds were determined using extensive nuclear magnetic resonance and mass spectrometry spectroscopic analyses, and the absolute configuration of compound 1 was determined using Marfey's method. Bioactivity assays revealed that compounds 1 and 2 exhibited significant neuroprotective properties against glutamate-induced PC12 cell death at concentrations ranging from 0.02 to 0.31 μM.