Design of supramolecular hybrid nanomaterials comprising peptide-based supramolecular nanofibers and in situ generated DNA nanoflowers through rolling circle amplification

The artificial construction of multicomponent supramolecular materials comprising plural supramolecular architectures that are assembled orthogonally from their constituent molecules has attracted growing attention. Here, we describe the design and development of multicomponent supramolecular materials by combining peptide-based self-assembled fibrous nanostructures with globular DNA nanoflowers constructed by the rolling circle amplification reaction. The orthogonally constructed architectures were dissected by fluorescence imaging using the selective fluorescence staining procedures adapted to this study. The present, unique hybrid materials developed by taking advantage of each supramolecular architecture based on their peptide and DNA functions may offer distinct opportunities to explore their bioapplications as a soft matrix.

Automated summary

The artificial construction of multicomponent supramolecular materials has attracted attention due to their orthogonally assembled architectures. In this study, we combined peptide-based self-assembled fibrous nanostructures with globular DNA nanoflowers to develop unique hybrid materials. Fluorescence imaging was used to dissect the orthogonally constructed architectures. By utilizing the distinct functions of peptides and DNA, these materials show promising opportunities for bioapplications as a soft matrix.