Using a Madurella mycetomatis-specific PCR on grains obtained via non-invasive fine-needle aspirated material is more accurate than cytology

Article Dermatology

Comparing the performance of the common used eumycetoma diagnostic tests

Emmanuel Edwar Siddig et al.

Summary: This study compared the diagnostic performance of ultrasound, histopathology, culture, and two species-specific PCRs in identifying the causative organism of mycetoma. The results showed that the two PCR methods were the most sensitive and specific, while ultrasound was the least specific. The evaluation concluded that PCR directly on DNA isolated from grains is the most rapid and reliable diagnostic tool.

MYCOSES (2023)

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Article Parasitology

The developed molecular biological identification tools for mycetoma causative agents: An update

Emmanuel Edwar Siddig et al.

Summary: Mycetoma can be caused by bacteria or fungi, resulting in actinomycetoma or eumycetoma. Molecular techniques, such as MALDI-TOF MS and DNA sequencing, enable rapid and accurate identification of causative organisms for guiding patient management and treatment.

ACTA TROPICA (2022)

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Article Dermatology

Ultrasound-guided fine-needle aspiration cytology significantly improved mycetoma diagnosis

E. E. Siddig et al.

Summary: Ultrasound-guided fine-needle aspiration cytology (US-FNAC) has higher diagnostic accuracy than conventional FNAC for the diagnosis of mycetoma, allowing for rapid differentiation between the two types of mycetoma.

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY (2022)

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Article Public, Environmental & Occupational Health

In vitro susceptibility testing for black grain eumycetoma causative agents

Wendy W. J. van de Sande

Summary: Eumycetoma is a neglected tropical disease characterized by subcutaneous swellings caused by fungal infection. In vitro susceptibility assays have been developed for causative agents, mainly used for determining inhibition of growth by antifungal agents. However, a clear correlation between in vitro inhibition and therapeutic efficacy has not been established, requiring further research.

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE (2021)

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Article Microbiology

Eumycetoma Medical Treatment: Past, Current Practice, Latest Advances and Perspectives

Emmanuel Edwar Siddig et al.

Summary: Mycetoma, a neglected tropical disease prevalent in Africa, is characterized by a prolonged disease duration and low cure rate. Effective management of eumycetoma depends on accurate diagnosis, identification of the causative agent, and evaluating susceptibility to available drugs. The review discusses currently available antifungal agents and optimizing newly developed antifungals for potential second-line treatment of eumycetoma.

MICROBIOLOGY RESEARCH (2021)

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Article Infectious Diseases