Co-Fermentation of Glucose-Xylose Mixtures from Agroindustrial Residues by Ethanologenic Escherichia coli: A Study on the Lack of Carbon Catabolite Repression in Strain MS04

The production of biofuels, such as bioethanol from lignocellulosic biomass, is an important task within the sustainable energy concept. Understanding the metabolism of ethanologenic microorganisms for the consumption of sugar mixtures contained in lignocellulosic hydrolysates could allow the improvement of the fermentation process. In this study, the ethanologenic strain Escherichia coli MS04 was used to ferment hydrolysates from five different lignocellulosic agroindustrial wastes, which contained different glucose and xylose concentrations. The volumetric rates of glucose and xylose consumption and ethanol production depend on the initial concentration of glucose and xylose, concentrations of inhibitors, and the positive effect of acetate in the fermentation to ethanol. Ethanol yields above 80% and productivities up to 1.85 g(EtOH)/Lh were obtained. Furthermore, in all evaluations, a simultaneous co-consumption of glucose and xylose was observed. The effect of deleting the xyIR regulator was studied, concluding that it plays an important role in the metabolism of monosaccharides and in xylose consumption. Moreover, the importance of acetate was confirmed for the ethanologenic strain, showing the positive effect of acetate on the co-consumption rates of glucose and xylose in cultivation media and hydrolysates containing sugar mixtures.

Automated summary

This study investigated the metabolism of ethanologenic microorganisms for the consumption of sugar mixtures in lignocellulosic hydrolysates, with the aim of improving the fermentation process for the production of bioethanol. The results showed that the rates of glucose and xylose consumption, as well as ethanol production, were influenced by the initial concentrations of glucose and xylose, concentrations of inhibitors, and the positive effect of acetate. The study also highlighted the importance of the xyIR regulator in monosaccharide metabolism and xylose consumption, and confirmed the positive effect of acetate on the co-consumption rates of glucose and xylose.