4.6 Review

Impact of N-Linked Glycosylation on Therapeutic Proteins

Related references

Note: Only part of the references are listed.
Review Chemistry, Multidisciplinary

Strategies for Glycoengineering Therapeutic Proteins

Kris Dammen-Brower et al.

Summary: This article discusses the important role of glycosylation in therapeutic proteins and provides examples of drugs that have been improved through glycoengineering. As glycoengineering strategies develop, deliberate modification of therapeutic protein glycosylation will become more prevalent.

FRONTIERS IN CHEMISTRY (2022)

Article Biology

An embeddable molecular code for Lewis X modification through interaction with fucosyltransferase 9

Taiki Saito et al.

Summary: A 29-amino acid sequence in the N-terminal domain of LAMP-1 promotes its Lewis X glycosylation and can be embedded into other proteins for Lewis X glycoengineering, opening up new possibilities for protein and cell engineering.

COMMUNICATIONS BIOLOGY (2022)

Article Biochemistry & Molecular Biology

O-glycosylation and its role in therapeutic proteins

Nicole Thompson et al.

BIOSCIENCE REPORTS (2022)

Article Immunology

New Opportunities in Glycan Engineering for Therapeutic Proteins

Xiaotian Zhong et al.

Summary: Glycans play important roles in cellular and biological functions as vital metabolic, structural, and physiological regulators. Recent research in glycoengineering, focusing on the conjugates of N-acetyl-galactosamine and mannose-6-phosphate for lysosomal degradation, Fab glycans for antibody diversification, and sialylation therapeutic modulations and O-linked applications, has sparked continued interest in the field. The advancements in human glycome and the comprehensive network of glycosylation pathways present new opportunities for designing next-generation therapeutic proteins.

ANTIBODIES (2022)

Article Biochemistry & Molecular Biology

Rational engineering of an erythropoietin fusion protein to treat hypoxia

Jungmin Lee et al.

Summary: The fusion protein developed in this study stimulates red blood cell production and neuroprotection, while avoiding thrombosis. It is composed of an anti-glycophorin A nanobody and an erythropoietin mutant. The protein exhibited promising activity in vitro and in mouse models, highlighting its potential as a novel therapeutic molecule for hypoxia.

PROTEIN ENGINEERING DESIGN & SELECTION (2021)

Article Biochemistry & Molecular Biology

Glycosylation decreases aggregation and immunogenicity of adalimumab Fab secreted from Pichia pastoris

Hitomi Nakamura et al.

Summary: The study showed that glycosylation affects Fab aggregation and immunogenicity, leading to a reduction in elimination half-life in vivo. Glycoengineering has the potential to optimize therapeutic proteins for improved efficacy.

JOURNAL OF BIOCHEMISTRY (2021)

Review Mathematical & Computational Biology

Post-translational modifications in proteins: resources, tools and prediction methods

Shahin Ramazi et al.

Summary: Posttranslational modifications (PTMs) involve modifications to amino acid side chains in proteins after biosynthesis, affecting various aspects of protein functions. Disruptions in PTMs can lead to diseases, emphasizing the need for computational methods to predict PTMs. High-throughput experimental methods for PTM discovery are laborious, prompting the exploration of computational tools and databases to advance research in this area.

DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION (2021)

Review Chemistry, Multidisciplinary

In Vivo Stability of Therapeutic Proteins

Joachim Schuster et al.

PHARMACEUTICAL RESEARCH (2020)

Review Chemistry, Applied

N-Glycosylation of IgG and IgG-Like Recombinant Therapeutic Proteins: Why Is It Important and How Can We Control It?

Natalia I. Majewska et al.

ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 11 (2020)

Article Biochemistry & Molecular Biology

Enhancing the stability of adalimumab by engineering additional glycosylation motifs

Mouhamad Reslan et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Review Biotechnology & Applied Microbiology

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

B. Owczarek et al.

BIOMED RESEARCH INTERNATIONAL (2019)

Review Biochemistry & Molecular Biology

Glycoengineered antibodies: towards the next-generation of immunotherapeutics

Renato Mastrangeli et al.

GLYCOBIOLOGY (2019)

Review Cell Biology

IgG Fc engineering to modulate antibody effector functions

Xinhua Wang et al.

PROTEIN & CELL (2018)

Article Medicine, Research & Experimental

Analytical similarity assessment of rituximab biosimilar CT-P10 to reference medicinal product

Kyoung Hoon Lee et al.

Article Multidisciplinary Sciences

Adaptive antibody diversification through N-linked glycosylation of the immunoglobulin variable region

Fleur S. van de Bovenkamp et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Review Biotechnology & Applied Microbiology

Impact of host cell line choice on glycan profile

Justin Bryan Goh et al.

CRITICAL REVIEWS IN BIOTECHNOLOGY (2018)

Article Pharmacology & Pharmacy

N-glycosylation heterogeneity and the influence on structure, function and pharmacokinetics of monoclonal antibodies and Fc fusion proteins

Fabian Higel et al.

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2016)

Review Immunology

The Emerging Importance of IgG Fab Glycosylation in Immunity

Fleur S. van de Bovenkamp et al.

JOURNAL OF IMMUNOLOGY (2016)

Review Biochemistry & Molecular Biology

Fc glycans of therapeutic antibodies as critical quality attributes

Dietmar Reusch et al.

GLYCOBIOLOGY (2015)

Review Chemistry, Multidisciplinary

Asialoglycoprotein receptor mediated hepatocyte targeting - Strategies and applications

Anisha A. D'Souza et al.

JOURNAL OF CONTROLLED RELEASE (2015)

Article Medicine, Research & Experimental

Consistency of quality attributes for the glycosylated monoclonal antibody Humira® (adalimumab)

Paul W. Tebbey et al.

Article Chemistry, Multidisciplinary

Molecular-scale features that govern the effects of O-glycosylation on a carbohydrate-binding module

Xiaoyang Guan et al.

CHEMICAL SCIENCE (2015)

Review Biochemistry & Molecular Biology

N-linked protein glycosylation in the ER

Markus Aebi

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2013)

Article Biochemistry & Molecular Biology

NMR analysis demonstrates immunoglobulin G N-glycans are accessible and dynamic

Adam W. Barb et al.

NATURE CHEMICAL BIOLOGY (2011)

Review Cell Biology

Erythropoietin: A Hormone with Multiple Functions

Matilde Lombardero et al.

PATHOBIOLOGY (2011)

Review Biochemistry & Molecular Biology

Trends in Glycosylation, Glycoanalysis and Glycoengineering of Therapeutic Antibodies and Fc-Fusion Proteins

Alain Beck et al.

CURRENT PHARMACEUTICAL BIOTECHNOLOGY (2008)

Review Biotechnology & Applied Microbiology

Protein therapeutics: A summary and pharmacological classification

Benjamin Leader et al.

NATURE REVIEWS DRUG DISCOVERY (2008)

Article Multidisciplinary Sciences

Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG fc

Robert M. Anthony et al.

SCIENCE (2008)

Article Transplantation

Pharmacology of darbepoetin alfa

Iain C. Macdougall et al.

NEPHROLOGY DIALYSIS TRANSPLANTATION (2007)

Article Multidisciplinary Sciences

Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation

Yoshikatsu Kaneko et al.

SCIENCE (2006)

Review Chemistry, Medicinal

Glycoengineering: The effect of glycosylation on the properties of therapeutic proteins

AM Sinclair et al.

JOURNAL OF PHARMACEUTICAL SCIENCES (2005)

Review Pharmacology & Pharmacy

Rational design and engineering of therapeutic proteins

SA Marshall et al.

DRUG DISCOVERY TODAY (2003)

Article Biochemistry & Molecular Biology

Glycosylation of erythropoietin affects receptor binding kinetics: Role of electrostatic interactions

RJ Darling et al.

BIOCHEMISTRY (2002)

Article Multidisciplinary Sciences

Mannose receptor-mediated regulation of serum glycoprotein homeostasis

SJ Lee et al.

SCIENCE (2002)