Journal
MOLECULES
Volume 27, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/molecules27249038
Keywords
green chemistry; vandetanib; human urine; human plasma; human liver microsomes
Funding
- Deputyship for Research & Innovation, Ministry of Education, in Saudi Arabia [IFKSURG-1085]
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A rapid, sensitive and validated HPLC-UV method has been established for the quantification of VNB in pure human biological fluids and HLMs, and also applied to the metabolic stability assessment of VNB.
Vandetanib (Caprelsa (R); VNB) is a prescription medicine that is used for the treatment of medullary thyroid cancer that has disrupted other body parts or that cannot be removed by surgery. It is considered a tyrosine kinase inhibitor (TKI). Fast, sensitive and validated HPLC-UV was established for VNB quantification in pure human biological fluids (urine and plasma) and human liver microsomes (HLMs). This analytical methodology was applied also to the metabolic stability assessment of VNB. This method was performed using a phenyl column (250 mm x 4.6 mm id, 5 mu m particle size). A sodium dodecyl sulphate solution (0.05 M, pH 3.0 using 0.02 M orthophosphoric acid) containing 0.3% triethylamine and 10% n-butanol was used as a mobile phase and was pumped isocratically at a flow rate of 0.7 mL/min and at a 260 nm detection wavelength. The total elution time was 6 min with an injection volume of 20 mu L. The linearity of the established methodology ranged from 30 to 500 ng/mL in pure form and 50 to 500 ng/mL (r(2) >= 0.9994) in human biological fluids and HLMs. No significant interference from the matrix components was observed. The proposed methodology revealed the benefits of being green, reliable and economic.
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