4.6 Article

Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization

Journal

MOLECULES
Volume 27, Issue 23, Pages -

Publisher

MDPI
DOI: 10.3390/molecules27238479

Keywords

recombinant spider silk protein; dimensional stability; C-13 NMR spectroscopy

Funding

  1. JSPS KAKENHI
  2. [JP19K05609]

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In this study, spider silk was successfully applied in a practical way using C-13 solid-state NMR. Recombinant spider silk materials with high dimensional stability were produced through molecular design, making them suitable for textiles and biomaterials.
Spider dragline silk has unique characteristics of strength and extensibility, including supercontraction. When we use it as a biomaterial or material for textiles, it is important to suppress the effect of water on the fiber by as much as possible in order to maintain dimensional stability. In order to produce spider silk with a highly hydrophobic character, based on the sequence of ADF-3 silk, we produced recombinant silk (RSSP(VLI)) where all QQ sequences were replaced by VL, while single Q was replaced by I. The artificial RSSP(VLI) fiber was prepared using formic acid as the spinning solvent and methanol as the coagulant solvent. The dimensional stability and water absorption experiments of the fiber were performed for eight kinds of silk fiber. RSSP(VLI) fiber showed high dimensional stability, which is suitable for textiles. A remarkable decrease in the motion of the fiber in water was made evident by C-13 solid-state NMR. This study using C-13 solid-state NMR is the first trial to put spider silk to practical use and provide information regarding the molecular design of new recombinant spider silk materials with high dimensional stability in water, allowing recombinant spider silk proteins to be used in next-generation biomaterials and materials for textiles.

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