Journal
MOLECULES
Volume 27, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/molecules27238267
Keywords
ochratoxin a; aptamer; fluorescence; molecular beacon; sensor
Funding
- National Natural Science Foundation of China
- [22074156]
- [21874146]
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A simple molecular aptamer beacon (MAB) sensor for rapid detection of the carcinogenic fungal secondary metabolite OTA has been developed. The sensor showed high selectivity and sensitivity, enabling detection of OTA in a wide dynamic concentration range. The method demonstrated capability for OTA analysis in practical applications.
Ochratoxin A (OTA) is a carcinogenic fungal secondary metabolite which causes wide contamination in a variety of food stuffs and environments and has a high risk to human health. Developing a rapid and sensitive method for OTA detection is highly demanded in food safety, environment monitoring, and quality control. Here, we report a simple molecular aptamer beacon (MAB) sensor for rapid OTA detection. The anti-OTA aptamer has a fluorescein (FAM) labeled at the 5 ' end and a black hole quencher (BHQ1) labeled at the 3 ' end. The specific binding of OTA induced a conformational transition of the aptamer from a random coil to a duplex-quadruplex structure, which brought FAM and BHQ1 into spatial proximity causing fluorescence quenching. Under the optimized conditions, this aptamer sensor enabled OTA detection in a wide dynamic concentration range from 3.9 nM to 500 nM, and the detection limit was about 3.9 nM OTA. This method was selective for OTA detection and allowed to detect OTA spiked in diluted liquor and corn flour extraction samples, showing the capability for OTA analysis in practical applications.
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