Journal
MOLECULES
Volume 28, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/molecules28020675
Keywords
apple pomace; polyphenolic compounds; waste valorization; antioxidant activity; biological properties; cytocompatibility
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Apple pomace (AP) from the food industry contains bioactive polyphenolic compounds. This study focuses on the recovery and evaluation of these compounds from different fractions of AP. Subcritical water extraction showed higher total polyphenolic compounds (39.08 mg GAE/g db) compared to ethanol extraction (10.78 mg GAE/g db). HPLC analysis identified phloridzin, chlorogenic acid, and quercetin as the main polyphenolics in AP.
Apple pomace (AP) from the food industry is a mixture of different fractions containing bioactive polyphenolic compounds. This study provides a systematic approach toward the recovery and evaluation of the physiochemical and biological properties of polyphenolic compounds from AP. We studied subcritical water extraction (SCW) and solvent extraction with ethanol from four different AP fractions of pulp, peel, seed, core, and stem (A), peel (B), seed and core (C), and pulp and peel (D). The subcritical water method at the optimum condition resulted in total polyphenolic compounds (TPC) of 39.08 +/- 1.10 mg GAE per g of AP on a dry basis compared to the ethanol extraction with TPC content of 10.78 +/- 0.94 mg GAE/g db. Phloridzin, chlorogenic acid, and quercetin were the main identified polyphenolics in the AP fractions using HPLC. DPPH radical scavenging activity of fraction B and subcritical water (SW) extracts showed comparable activity to ascorbic acid while all ethanolic extracts were cytocompatible toward human fibroblast (3T3-L1) and salivary gland acinar cells (NS-SV-AC). Our results indicated that AP is a rich source of polyphenolics with the potential for biomedical applications.
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