Journal
MOLECULES
Volume 27, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/molecules27248947
Keywords
Citrus sinensis; plant; macromolecules; proteomic
Funding
- CNPq-National Council for Scientific and Technological Development [CNPq-INCT: 573742/2008-1, CNPq-INCT: 465357/2014-8, 141596/2011-2, 445518/2014-6]
- FAPESP-Sao Paulo Research Foundation [FAPESP-INCT: 08/57859-5, FAPESP-Phd scholarship: 11/22975-8]
- CAPES-Higher Education Improvement Coordination
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Citrus canker, a disease that causes serious problems to the citrus industry, can be rapidly diagnosed using MALDI-TOF mass spectrometry combined with chemometric analysis.
Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a disease that causes serious problems to the global citrus industry. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS) has been used in human medicine to diagnose various diseases caused by both fungi and bacteria. In agriculture, this technique has potential for the diagnosis of diseases due to the low cost of large-scale analysis and quickness. This study showed that MALDI-TOF MS combined with chemometric analysis was effective for differentiating the macromolecule profile of orange leaves with canker lesions, healthy leaves, and leaves with phytotoxicity symptoms, proving that this technique may be used for the rapid diagnosis of citrus canker.
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