Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

Automated summary

Naturally occurring ribozymes with a modular architecture can serve as promising platforms for constructing RNA nanostructures, as their modular redesign allows for oligomerization. These resulting RNA nanostructures can retain the catalytic function of the parent ribozyme, depending on their assembly. In this study, open-form oligomers of a bimolecular form of an RNase P ribozyme were designed and constructed, and analyzed biochemically and by atomic force microscopy (AFM).