Journal
MOLECULES
Volume 27, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/molecules27248878
Keywords
biofilms; exoenzymes; Pectobacterium carotovorum subsp; carotovorum; quorum-sensing inhibitor; rhapontigenin; keeping vegetables fresh
Funding
- Science and Technology Planning Project of Changsha [kq2107022]
- Changsha-Zhuzhou-Xiangtan Landmark Project [2019XK2002]
- Key Research & Development Plan of Hunan Province [2021NK2014]
- Natural Science Foundation of Hunan Province
- Natural Science Foundation of Changsha [kq2202224]
- third batch of the major Scientific Research Projects of Hunan Agricultural University (Innovation Team Cultivation Project 2019)
- Double First-Class Discipline Construction Project of Hunan Agricultural University [SYL2019061]
- 2022 Innovative Research Project for Postgraduate Students of Hunan Province [CX20220683]
- 2021 Innovative Research Project for Postgraduate Students of Hunan Agricultural University [2021-63]
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This study evaluated the potential of rhapontigenin as a quorum sensing (QS) inhibitor against Pectobacterium carotovorum subsp. carotovorum (P. carotovorum). The results show that rhapontigenin significantly inhibited the motility, EPS production, biofilm formation, virulence-exoenzyme synthesis and AHL production of P. carotovorum. Moreover, direct-inoculation assay revealed that rhapontigenin could extend the shelf-life of vegetables and prevent the spread of soft-rot symptoms.
The aim of this study was to devise a method to protect Chinese cabbage (Brassica chinensis) and lettuce (Lactuca sativa) from bacterial-disease-induced damage during storage. Thus, the potential of rhapontigenin as a quorum sensing (QS) inhibitor against Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) was evaluated. The QS inhibitory effects of rhapontigenin were confirmed by significant inhibition of the production of violacein in Chromobacterium violaceum CV026 (C. violaceum, CV026). The inhibitory effects of rhapontigenin on the motility, exopolysaccharide (EPS) production, biofilm formation and virulence-exoenzyme synthesis of P. carotovorum were investigated. Acyl-homoserine lactones (AHLs) were quantified using liquid chromatography-mass spectrometry (LC-MS). The inhibitory effects of rhapontigenin on the development of biofilms were observed using fluorescence microscopy and scanning electron microscopy (SEM). A direct-inoculation assay was performed to investigate the QS inhibitory effects of rhapontigenin on P. carotovorum in Chinese cabbage and lettuce. Our results demonstrated that rhapontigenin exhibited significant inhibition (p < 0.05) of the motility, EPS production, biofilm formation, virulence-exoenzyme synthesis and AHL production of P. carotovorum. Additionally, the result of the direct-inoculation assay revealed that rhapontigenin might provide vegetables with significant shelf-life extension and prevent quality loss by controlling the spread of soft-rot symptoms. Consequently, the study provided a significant insight into the potential of rhapontigenin as a QS inhibitor against P. carotovorum.
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