Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 159, Issue -, Pages 133-141Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2016.03.007
Keywords
Tertiary pyridine; DNA binding; Partial intercalation; Docking; Copper complexes
Categories
Funding
- CSIR project [01(2742)/13/EMR-II]
Ask authors/readers for more resources
Copper based metal complexes have been studied extensively towards DNA interaction aspects, the possible interactions being at the major or minor grooves, intercalation between base pairs, etc. The nature of the ligand decides the binding mode of the complexes thereby exerting different biological significance. Based on this, we have synthesized two mixed ligand copper(II) complexes, [Cu(meFtpy)(bpy)](NO3)(2.2)(H2O) (1) and [Cu(meFtpy)(phen)](NO3)(2)center dot(H2O) (2) based on new furfuryl substituted tertiary pyridine ligand (meFtpy) and ancillary ligands (phen, bpy). They are characterized by UV Visible, FT-IR, 1H &13C NMR and mass spectroscopic techniques. The structures of both the complexes are confirmed by single crystal XRD revealing triclinic crystal system showing penta coordination possessing distorted square pyramidal geometry. The binding ability of the complexes has been explored-based on the results of DNA binding studies assessed by different spectroscopic techniques like UV absorption titration, fluorescence displacement assay and circular dicroism and found to show partial intercalative behaviour. The binding constant (K-b) values as obtained from UV absorption titration are found to be 1.29 x 10(4) M-1 for 1 and 1.46 x 10(4) M-1 for 2 and are compared with the values obtained for doxorubicin, a partial intercalator drug. The binding affinities obtained from absorption titration are found in the order as K-b(doxo) > K-b(2) > K-b(1) > K-b(meFtpy). (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available