Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 165, Issue -, Pages 51-57Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2016.10.016
Keywords
TMPyP; MRSA; E. coli; PDI; Singlet oxygen; Metalloporphyrin
Categories
Funding
- Student Opportunity Funds
- Department of Chemical and Biological Sciences at Rockford Univesity
- Aqua Aerobics in Rockford, IL, USA
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Increasing rates of antibiotic resistance coupled with the lack of novel antibiotics threatens proper clinical treatment and jeopardizes their use in prevention. A photodynamic approach appears to be an innovative treatment option, even for multi-drug resistant strains of bacteria. Three components are utilized in photodynamic inactivation: a photosensitizer, light source, and oxygen. Variations in photosensitizers strongly influence microbial binding and bactericidal activity. In this study, four different cationic metalloporphyrins (Cu2+, Fe2+, Pd2+, Zn2+) were compared to the free-base ligand 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porPhyrin regarding their electronic properties and generation of reactive oxygen species upon subsequent 405 nm violet-blue irradiation. Staphylococcus aureus and Escherichia coli were used as representatives of Gram-positive and-negative, respectively, to assess bactericidal effects by the photodynamic process. Bacterial cultures were pre-incubated with porphyrins and exposed to varying doses of 405 nm irradiation (0-30 J/cm(2)). Metalloporphyrins containing Cu2+ and Fe2+ demonstrated minimal effects on viability. Pronounced bactericidal activity was evident with free-base ligand, Zn2+, and Pd2+; though significantly stronger effects were apparent with Pd2+. Photodynamic killing was directly proportional to reactive oxygen species production post-illumination. These data provide new insight into the influence of metal chelation on photosensitizer activity on bactericidal singlet oxygen production. The strong anti-microbial photodynamic action through the use of a portable light-emitting diode over short time intervals (seconds) provides support for its potential use in self-treatment. (C) 2016 The Authors. Published by Elsevier B.V.
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