Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 478, Issue 9, Pages 1915-1925Publisher
SPRINGER
DOI: 10.1007/s11010-022-04626-7
Keywords
Antioxidant; Asterarcys quadricellulare; Cardiovascular diseases; Caspase-3; High value products; Reactive Oxygen Species
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Oxidative stress is a significant factor in cardiovascular diseases, and finding effective antioxidant molecules is crucial for disease prevention. This study evaluated the potential of microalgae bioactive compounds in protecting H9c2 cardiomyoblasts from H2O2-induced oxidative stress. Four microalgal species were tested for their antioxidant capacity, and the hexanoic extracts showed high antioxidant activity. Among them, Asterarcys quadricellulare exhibited the highest antioxidant capacity and protected against H2O2-induced oxidative stress similar to N-Acetylcysteine. The extract also showed anti-apoptotic properties. These findings suggest that A. quadricellulare could be a natural and efficient antioxidant for preventing cardiovascular diseases.
Oxidative stress has recently been identified as an important mediator of cardiovascular diseases. The need to find efficient antioxidant molecules is essential in the disease's prevention. Therefore, the present study aimed to evaluate the potential of microalgae bioactive in protecting H9c2 cardiomyoblasts from H2O2-induced oxidative stress. Four microalgal species were investigated for their antioxidant capacity. A qualitative assessment of oxidative stress in H9c2 cardiomyoblasts stained with DCFH-DA, treated with the highly active microalgae extracts, was performed. The protein expression of total caspase-3 was also examined to investigate whether the extract protects H9c2 cardimyoblasts from H2O2-induced apoptosis. High antioxidant activity was observed for the hexanoic extracts after 10 days of cultivation. Asterarcys quadricellulare exhibited the highest antioxidant capacity of 110.59 & PLUSMN; 1.75 mg TE g(-1) dry weight and was tested against H9c2 cardiomyoblasts, which were initially subjected to H2O2-induced oxidative stress. This hexanoic extract protected against H2O2 induced oxidative stress with a similar scavenging capacity as N-Acetylcysteine. Furthermore, total caspase-3 was increased following treatment with the hexanoic extract, suggesting that A. quadricellulare also had anti-apoptotic properties. The outcome of our study highlighted the possible use of the local A. quadricellulare strain QUCCCM10 as a natural, safe, and efficient antioxidant to prevent cardiovascular diseases.
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