Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 478, Issue 8, Pages 1887-1898Publisher
SPRINGER
DOI: 10.1007/s11010-022-04643-6
Keywords
Collagen homeostasis; ECM disorganization; Hypercholesterolemia; Hyperlipidemia; Rotator cuff tendon injury
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The impact of hyperlipidemia on rotator cuff tendon injury (RCTI) is still unclear. This study found that hyperlipidemia caused changes in major extracellular matrix components in tendon tissues and tenocytes. It also affected the expression levels of certain proteins and promoted lipid uptake and the generation of cellular reactive oxygen radicals. Understanding these mechanisms may provide new approaches for managing RCTI.
Hyperlipidemia is an important risk factor in the development and progression of tendon pathology, however its role in aggravating rotator cuff tendon injury (RCTI) is largely unknown. We aimed to assess the expression status of key extracellular matrix (ECM) components in the tendon tissues and tenocytes under hyperlipidemia. Shoulder rotator cuff (RC) tendon tissues harvested from the swine model of hyperlipidemia displayed alterations in histomorphometry and the expression status of major ECM component proteins including COL-I, COL-III, COL-IV, COL-V, COL-VI, MMP2, and MMP9. Similarly, the LDL- and oxLDL-challenged tenocytes displayed altered expression of the same proteins at both transcriptional and translational levels. In addition, the lipid uptake and cellular reactive oxygen radicals predominated in the lipid-challenged tenocytes compared to the control. Overall, the LDL-treated cells displayed predominant pathological alterations compared to the ox-LDL-treated cells. Further understanding regarding the underlying molecular mechanisms driving the tendon matrisome alteration and subsequent aggravated RCTI pathology in hyperlipidemia could open novel translational avenues in the management of RCTI.
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