Evaluation of antibacterial activity of plant mediated CaO nanoparticles using Cissus quadrangularis extract

An assessment of antibacterial activity of greenly synthesized nanoparticles using aqueous stem extract of Cissus quadrangularis was carried out. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, SEM, XRD, FTIR and further subjected for antibacterial activity against the pathogens Staphylococcus aureus, Escherichia colt, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae and Vibrio cholerae. The SEM photograph represents cubic and hexagonal shape of NPs about 58 nm respectively whereas the XRD indicated the pure phase of the product and no impurity in peaks of well crystallized products. The FTIR spectrum of nanoparticles showed intensive peaks with blue shift indicating the crystalline and shorten the distance of crystal lattice. The plant mediated CaO nanopartides showed maximum inhibition on E. colt followed by other strains. In MIC, the plant mediated CaO NPs possess high activity against all the test organisms whereas the CaCl2 and CaO compounds were moderately active. The approach to the synthesis of plant mediated CaO NPs has many advantages as scaled up, economic viability, etc. Application of such ecofriendly nanoparticles in bactericidal, wound healing and other medical applications makes this method potential existing for the large scale synthesis of the inorganic materials. (C) 2015 Elsevier B.V. All rights reserved.