Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 68, Issue 7, Pages 901-911Publisher
OXFORD UNIV PRESS
DOI: 10.1111/jphp.12544
Keywords
apoptosis cordycepin; lung cancer; migration; proliferation
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ObjectivesOur study aimed to evaluate the effect of cordycepin on human lung cancer cell lines A549 and NCI-H460. MethodsHuman lung cancer A549 cells and NCI-H460 cells were treated with different concentrations of cordycepin for different times. Cells incubated without cordycepin were defined as a control. The cell proliferation, migration and apoptosis were, respectively, determined by MTT assay, transwell migration assay and flow cytometry. Additionally, the expression levels of related proteins associated with cell cycle, epithelial-mesenchymal transition (EMT) and apoptosis were examined. Key findingsThe survival rate of A549 cells and NCI-H460 cells treated with cordycepin significantly decreased compared with untreated cells in a concentration-dependent manner, while the apoptosis rate increased. The migration number of cells treated with cordycepin significantly decreased as the increase in concentration. qRT-PCR and Western blot analysis showed that the aberrant expression of related molecules associated with cell cycle, migration and apoptosis was observed in the lung cancer cells, such as cyclin B, cyclin E, MMP-9, caspase-3 and Bcl-2. ConclusionsCordycepin may exert inhibitory effects on the development of human lung cancer via inhibiting cell proliferation, suppressing migration and inducing apoptosis, suggesting that cordycepin may have therapeutic potential for the treatment of this disease.
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