Journal
MARINE DRUGS
Volume 21, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/md21020076
Keywords
Roseofilum; looekeyolide; malyngamide; lasso peptide; metagenome-assembled genomes; biosynthetic gene cluster
Categories
Ask authors/readers for more resources
Black band disease is a globally distributed and easily recognizable coral disease. Despite years of study, the etiology of this disease is not completely understood. Through chemical ecology and metagenomic sequencing, unique strains of Roseofilum species were discovered from Siderastrea siderea corals, which differ from other corals in the Caribbean and Pacific. These strains have the genetic capacity to produce lasso peptides, suggesting their potential role in disease etiology.
Black band disease is a globally distributed and easily recognizable coral disease. Despite years of study, the etiology of this coral disease, which impacts dozens of stony coral species, is not completely understood. Although black band disease mats are predominantly composed of the cyanobacterial species Roseofilum reptotaenium, other filamentous cyanobacterial strains and bacterial heterotrophs are readily detected. Through chemical ecology and metagenomic sequencing, we uncovered cryptic strains of Roseofilum species from Siderastrea siderea corals that differ from those on other corals in the Caribbean and Pacific. Isolation of metabolites from Siderastrea-derived Roseofilum revealed the prevalence of unique forms of looekeyolides, distinct from previously characterized Roseofilum reptotaenium strains. In addition, comparative genomics of Roseofilum strains showed that only Siderastrea-based Roseofilum strains have the genetic capacity to produce lasso peptides, a family of compounds with diverse biological activity. All nine Roseofilum strains examined here shared the genetic capacity to produce looekeyolides and malyngamides, suggesting these compounds support the ecology of this genus. Similar biosynthetic gene clusters are not found in other cyanobacterial genera associated with black band disease, which may suggest that looekeyolides and malyngamides contribute to disease etiology through yet unknown mechanisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available