Journal
MARINE DRUGS
Volume 20, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/md20110700
Keywords
marine bacteria; deep-sea; Idiomarina zobellii; lipooligosaccharide; lipid A; NMR spectroscopy; MALDI mass spectrometry
Categories
Funding
- Ministry of Science and Higher Education, Russian Federation [13.1902.21.0012 (075-15-2020-796)]
Ask authors/readers for more resources
This article describes a structural study of the R-type lipopolysaccharide isolated from a deep-sea marine Gram-negative bacterium, revealing a novel and unique pentasaccharide skeleton. The study was conducted using a multidisciplinary approach including chemical analyses, NMR spectroscopy, and MALDI mass spectrometry.
The structural characterization of lipopolysaccharides has critical implications for some biomedical applications, and marine bacteria are an inimitable source of new glyco-structures potentially usable in medicinal chemistry. On the other hand, lipopolysaccharides of marine Gram-negative bacteria present certain structural features that can help the understanding of the adaptation processes. The deep-sea marine Gram-negative bacterium Idiomarina zobellii KMM 231(T), isolated from a seawater sample taken at a depth of 4000 m, represents an engaging microorganism to investigate in terms of its cell wall components. Here, we report the structural study of the R-type lipopolysaccharide isolated from I. zobellii KMM 231(T) that was achieved through a multidisciplinary approach comprising chemical analyses, NMR spectroscopy, and MALDI mass spectrometry. The lipooligosaccharide turned out to be characterized by a novel and unique pentasaccharide skeleton containing a very short mono-phosphorylated core region and comprising terminal neuraminic acid. The lipid A was revealed to be composed of a classical disaccharide backbone decorated by two phosphate groups and acylated by i13:0(3-OH) in amide linkage, i11:0 (3-OH) as primary ester-linked fatty acids, and i11:0 as a secondary acyl chain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available