Journal
JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 105, Issue 8, Pages 2338-2348Publisher
WILEY
DOI: 10.1016/j.xphs.2016.06.010
Keywords
gels; IgG antibody; monoclonal antibody; protein formulation; proteins; rheology; salts/salt selection; colloid; stability
Funding
- MedImmune (Gaithersburg, MD)
Ask authors/readers for more resources
In this study, we investigated the effect of ionic strength (1.25-231 mM) on viscoelastic interfacial gels formed by a monoclonal antibody at silicone oil-water interfaces, and the formation of subvisible particles due to rupture of these gels. Rates of gel formation and their elastic moduli did not vary significantly with ionic strength. Likewise, during gel rupture no significant effects of ionic strength were observed on particle formation and aggregation as detected by microflow imaging, resonance mass measurement, and size exclusion chromatography. Subvisible particles formed by mechanical rupturing of the gels agglomerated over time, even during quiescent incubation, due to the colloidal instability of the particles. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available