Journal
LABORATORY INVESTIGATION
Volume 103, Issue 2, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.labinv.2022.100013
Keywords
amyotrophic lateral sclerosis; pig; swine; SOD1; misfolded protein
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In this study, a pig model of human mutant SOD1-mediated familial ALS was generated by delivering a genetic construct into porcine zygotes using intra-cytoplasmic sperm injection-mediated gene transfer. The established ALS pig model exhibited initial abnormalities of motor neurons and accumulated misfolded SOD1. This ALS pig model, with a body size similar to that of human beings, could be utilized for cell and gene therapy platforms in preclinical translational research.
Amyotrophic lateral sclerosis (ALS) causes progressive degeneration of the motor neurons. In this study, we delivered the genetic construct including the whole locus of human mutant superoxide dismutase 1 (SOD1) with the promoter region of human SOD1 into porcine zygotes using intra-cytoplasmic sperm injection-mediated gene transfer, and we thereby generated a pig model of human mutant SOD1-mediated familial ALS. The established ALS pig model exhibited an initial abnormality of motor neurons with accumulated misfolded SOD1. The ALS pig model, with a body size similar to that of human beings, will provide opportunities for cell and gene therapy platforms in preclinical translational research.(c) 2022 United States & Canadian Academy of Pathology. Published by Elsevier Inc. All rights reserved.
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