Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 120, Issue -, Pages 235-240Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2015.12.025
Keywords
Coptis chinensis Franch; In vitro AChE inhibition assay; UF-HPLC-ESI-Q-TOF/MS; Acetylcholinesterase inhibitor
Categories
Funding
- Natural Science Foundation of Shandong Province [ZR2013HL029]
- Science and Technology Development Foundation of Shandong Academy of Science [2012015]
- Program for Science and Technology Star for Jinan [2013030]
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In this study, an in vitro acetylcholinesterase (AChE) inhibition assay based on microplate reader combined with ultrafiltration high performance liquid chromatography-electrospray quadrupole time of flight mass (UF-HPLC-ESI-Q-TOF/MS) was developed for the rapid screening and identification of acetyl cholinesterase inhibitors (AChEI) from roots of Coptis chinensis Franch. Incubation conditions such as enzyme concentration, incubation time, incubation temperature and co -solvent was optimized so as to get better screening results. Five alkaloids including columbamine, jatrorrhizine, coptisine, palmatine and berberine were found with AChE inhibition activity in the 80% ethanol extract of C. chinensis Franch. The screened compounds were identified by HPLC-DAD-ESI-Q-TOF/MS compared with the reference stands and literatures. The screened results were verified by in vitro AChE inhibition assays, palmatine showed the best AChE inhibitory activities with IC50 values of 36.6 mu M among the five compounds. Results of the present study indicated that the combinative method using in vitro AChE inhibition assay and UF-HPLC-ESI-Q-TOF/MS could be widely applied for rapid screening and identification of AChEI from complex TCM extract. (C) 2015 Elsevier B.V. All rights reserved.
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