Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 125, Issue -, Pages 54-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2016.03.004
Keywords
Cocaine; Magnetic particles-based enzyme; immunoassay; Benzoylecgonine; Biological samples; Drugs of abuse; Pharmacokinetics
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Funding
- University of Zaragoza
- Bank of Santander [UZ2012-CIE-14]
- Spanish Science and Innovation Ministry [SERIBIO IPT-2011-1766-010000]
- Ministerio de Educacion, Cultura y Deporte [AP-2009-2648]
- Junta de Castilla y Leon [BU2212Al22]
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Cocaine is one of the most worldwide used illicit drugs. We report a magnetic particles-based enzyme linked immunoassay (mpEIA) method for the rapid and sensitive determination of cocaine (COC) in saliva, urine and serum samples. Under optimized conditions, the limits of detections were 0.09 ng mL(-1) (urine), 0.15 ng mL(-1) (saliva), and 0.06 ng mL(-1) COC (human serum). Sensitivities were in the range EC50 = 0.6-2.5 ng mL(-1) COC. The cross-reactivity with the principal metabolite benzoylecgonine (BZE) was only 1.6%. Recovering percentages of doped samples (0,10, 50, and 100 ng mL(-1) of COC) ranged from about 86-111%. Some advantages of the developed mpEIA over conventional ELISA kits are faster incubations, improved reproducibility, and consumption of lower amounts of antibody and enzyme conjugates due to the use of magnetic beads. The reported method was validated following the guidelines on bio-analytical methods of the European Medicines Agency (2011). Unmetabolized COC detection has a great interest in pharmacological, pharmacokinetics, and toxicokinetics studies, and can be used to detect a very recent COC use (1-6 h). (C) 2016 Elsevier B.V. All rights reserved.
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