Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 121, Issue -, Pages 84-90Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2016.01.012
Keywords
Pogostemon cablin; GC-FID; Quantitative analysis; Fingerprinting analysis; Chemometrics; Quality control
Categories
Funding
- Major Science and Technology Project of Hainan Province [ZDZX2013008]
- Modernization of Traditional Chinese Medicine of Hainan Province [2010ZY011]
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In this study, a simple, sensitive and reliable gas chromatography-flame ionization detection (GC-FID) method is established for quantitative chemical fingerprinting of essential oils from Pogostemon cablin. Oil samples are prepared by hydrodistillation, with yields ranging from 0.73% to 2.02%. The two main components of the oil, patchouli alcohol and pogostone, were detected simultaneously in 36 samples and were found to have average contents of 43.07% and 7.84%, respectively. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. All calibration curves showed excellent linearity (r(2) > 0.9992) within the test ranges, and the relative standard deviation (RSD) values for intraand inter-day precision were less than 1.5%, indicating a high degree of precision. The GC-FID chemical fingerprints of the 36 samples were established using 12 common peaks which account for over 90% of the total peak area. Chemometric techniques, including similarity analysis and hierarchical cluster analysis, were also employed to explore the similarities and outstanding consistencies among different P. cablin oil samples. The results demonstrate that chromatographic fingerprinting and quantitative analysis can be achieved simultaneously when evaluating quality and authenticating samples of P. cablin. (C) 2016 Elsevier B.V. All rights reserved.
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