Journal
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
Volume 27, Issue 2, Pages 489-499Publisher
SPRINGER
DOI: 10.1007/s10008-022-05330-8
Keywords
Electrochemical immunosensor; Ag-rGO nanocomposites; Biotin-streptavidin system; SARS-CoV-2; Receptor-binding domain
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An electrode platform immunosensor was developed for efficient and sensitive detection of SARS-CoV-2. The method showed high sensitivity, stability, and specificity, and has the potential to be used as a practical detection method for SARS-CoV-2.
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a grave threat to human life and health, it is essential to develop an efficient and sensitive detection method to identify infected individuals. This study described an electrode platform immunosensor to detect SARS-CoV-2-specific spike receptor-binding domain (RBD) protein based on a bare gold electrode modified with Ag-rGO nanocomposites and the biotin-streptavidin interaction system. The Ag-rGO nanocomposites was obtained by chemical synthesis and characterized by electrochemistry and scanning electron microscope (SEM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to record the electrochemical signals in the electrode modification. The differential pulse voltammetry (DPV) results showed that the limit of detection (LOD) of the immunosensor was 7.2 fg mL(-1) and the linear dynamic detection range was 0.015 similar to 158.5 pg mL(-1). Furthermore, this sensitive immunosensor accurately detected RBD in artificial saliva with favorable stability, specificity, and reproducibility, indicating that it has the potential to be used as a practical method for the detection of SARS-CoV-2.
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