Journal
JOURNAL OF PROTEOME RESEARCH
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.2c00714
Keywords
single-cell analysis; mass spectrometry; data-driven analysis; machine learning
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Improved throughput and lower detection limits have enabled single-cell chemical analysis to characterize different functional states of individual cells. Image-guided single-cell mass spectrometry leverages microscopy for high-throughput analysis of cellular targets. This study proposes DATSIGMA, a data-driven and machine learning workflow for enhanced interpretation of complex single-cell mass spectrometry data, which has been successfully tested on various biological applications, including brain cell classification. Due to its open-source nature, DATSIGMA allows for customization and adaptation to other types of single-cell mass spectrometry data.
Improved throughput of analysis and lowered limits of detection have allowed single-cell chemical analysis to go beyond the detection of a few molecules in such volume-limited samples, enabling researchers to characterize different functional states of individual cells. Image-guided single-cell mass spectrometry leverages optical and fluorescence microscopy in the highthroughput analysis of cellular and subcellular targets. In this work, we propose DATSIGMA (DAta-driven Tools for Single-cell analysis using Image-Guided MAss spectrometry), a workflow based on data-driven and machine learning approaches for feature extraction and enhanced interpretability of complex single-cell mass spectrometry data. Here, we implemented our toolset with user-friendly programs and tested it on multiple experimental data sets that cover a wide range of biological applications, including classifying various brain cell types. Because it is open-source, it offers a high level of customization and can be easily adapted to other types of single-cell mass spectrometry data.
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