Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 75, Issue 2, Pages 276-286Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jpp/rgac100
Keywords
3D printing; 5-FU; glaucoma; implant; drug delivery; fibrosis
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A sustained release 5-FU implant composed of PCL and CS was fabricated using 3D printing technology for preventing conjunctival fibrosis after glaucoma surgery. The implant released 5-FU steadily over 8 weeks and effectively suppressed fibroblast contractility. It exhibited biocompatibility, smooth surface, and degradability.
Objectives To develop a sustained release 5-fluorouracil (5-FU) implant by three-dimensional (3D) printing to effectively prevent conjunctival fibrosis after glaucoma surgery. Methods 3D-printed implants composed of polycaprolactone (PCL) and chitosan (CS) were fabricated by heat extrusion technology and loaded with 1% 5-FU. Light microscopy and scanning electron microscopy were used to study the surface morphology. The 5-FU concentration released over 8 weeks was measured by ultraviolet visible spectroscopy. The effects on cell viability, fibroblast contractility and the expression of key fibrotic genes were assessed in human conjunctival fibroblasts. Key findings The PCL-CS-5-FU implant sustainably released 5-FU over 8 weeks and the peak concentration was over 6.1 mu g/ml during weeks 1 and 2. The implant had a smooth surface and its total weight decreased by 3.5% after 8 weeks. The PCL-CS-5-FU implant did not affect cell viability in conjunctival fibroblasts and sustainably suppressed fibroblast contractility and key fibrotic genes for 8 weeks. Conclusions The PCL-CS-5-FU implant was biocompatible and degradable with a significant effect in suppressing fibroblast contractility. The PCL-CS-5-FU implant could be used as a sustained release drug implant, replacing the need for repeated 5-FU injections in clinic, to prevent conjunctival fibrosis after glaucoma surgery.
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