Atomic Force Microscopic Imaging of mRNA-lipid Nanoparticles in Aqueous Medium

Review Pharmacology & Pharmacy

Current Status and Challenges of Analytical Methods for Evaluation of Size and Surface Modification of Nanoparticle-Based Drug Formulations

Yuki Takechi-Haraya et al.

Summary: This review discusses the challenges and current status of analytical methods for evaluating the size and surface modifications of nanoparticle-based pharmaceutical products, such as liposomal drugs and new SARS-CoV-2 vaccines. The difficulties include measuring a wide range of solid-state nanoparticles, evaluating the sizes of polydisperse nanoparticles, and measuring non-spherical nanoparticles. Although some methods have been established for analyzing the surface modifications of nanoparticles, their applicability to nanoparticle-based products is unclear. The review also highlights the need for standardization in the size and surface measurements of nanoparticles, including the lack of available reference materials and the difficulty in selecting appropriate ones for modified nanoparticle surfaces. Future research and development in nanomedicine should focus on the practice and standardization of measurement methods to design surface modifications and ensure nanoparticle quality.

AAPS PHARMSCITECH (2022)

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Review Pharmacology & Pharmacy

The role of lipid components in lipid nanoparticles for vaccines and gene therapy

Camilla Hald Albertsen et al.

Summary: The translation discusses the important role of lipid nanoparticles in mRNA vaccines against COVID-19, as well as the contributions of lipid components to the LNP delivery platform. Challenges and strategies related to LNPs in treating various diseases are also explored.

ADVANCED DRUG DELIVERY REVIEWS (2022)

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Article Biophysics

Encapsulation state of messenger RNA inside lipid nanoparticles

Mark L. Brader et al.

Summary: Understanding the structure and microenvironment of mRNA within lipid nanoparticles is crucial for unlocking their biomedical potential. By using the permeating cationic dye thionine as a contrasting agent, cryo-electron microscopy images reveal the location of mRNA within the nanoparticles, suggesting it may be in solvent-filled cavities or associated with lipids.

BIOPHYSICAL JOURNAL (2021)

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Article Chemistry, Multidisciplinary