Chemo- and enantio-selective reversed-phase HPLC analysis of rosuvastatin using a cellulose-based chiral stationary phase in gradient elution mode

A direct reversed-phase high-performance liquid chromatographic (HPLC) method was developed for deter-mining the content of the enantiomeric impurity of the chiral statin rosuvastatin calcium salt (RSV) in com-mercial tablets. The baseline enantioseparation was achieved using the Lux Cellulose-2 column and a binary linear gradient of acetonitrile and trifluoroacetic acid 0.05% in an aqueous solution. The flow rate of the mobile phases and column temperature were set at 1.0 mL min- 1 and 40 degrees C, respectively. In comparison with the isocratic HPLC method reported in the European Pharmacopoeia (EP) monograph for RSV, the gradient elution method offered improved chemo-and enantio-selectivity and reduced analysis times. The limits of quantitation and detection of the enantiomeric impurity were found to be 0.15 and 0.05 mu g mL-1.

Automated summary

A direct reversed-phase high-performance liquid chromatographic method was developed for determining the content of the enantiomeric impurity of rosuvastatin calcium salt in tablets. The method offered improved selectivity and reduced analysis times compared to the method in European Pharmacopoeia. The limits of quantitation and detection of the enantiomeric impurity were found to be 0.15 and 0.05 μg/mL.