Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. Isolated from the Aquatic Environment, South Korea

Two novel bacterial species CJ51(T) and CJ63(T) belonging to the genus Chryseobacterium were isolated from the Upo wetland and the Han River, South Korea, respectively. Cells of these strains were Gram-stain-negative, aerobic, non-motile, rod-shaped, and catalase- and oxidase-positive. Both strains were shown to grow optimally at 30 degrees C and pH 7 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ51(T) and CJ63(T) belonged to the genus Chryseobacterium and were most closely related to Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316s(T) with 98.47% and 98.46% 16S rRNA sequence similarities, respectively. The average nucleotide identity values of strains CJ51(T) and CJ63(T) with its closely related type strains Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316s(T) were 81.9% and 82.1%, respectively. The major fatty acids of strains CJ51(T) and CJ63(T) were iso-C-15:0, iso-C-17:0 3-OH and summed feature 9 (C-16:0 10-methyl and/or iso-C-17:1 omega 9c). Menaquinone 6 (MK-6) was identified as the primary respiratory quinone in both strains. The major polar lipids of strains CJ51(T) and CJ63(T) were phosphatidylethanolamine and several unidentified amino lipids and lipids. Based on polyphasic taxonomy data, strains CJ51(T) and CJ63(T) represent novel species of the genus Chryseobacterium, for which names Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. are proposed respectively. The type strains are CJ51(T) (= KACC 22749(T) = JCM 35632(T)) and CJ63(T) (= KACC 22750(T) = JCM 35633(T)).

Automated summary

Two novel bacterial species, CJ51(T) and CJ63(T), were isolated from different locations in South Korea. They belong to the genus Chryseobacterium and have similar 16S rRNA sequences with other closely related species. These strains represent new species named Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. based on polyphasic taxonomy data.