Optimized prime editing efficiently generates heritable mutations in maize

Article Biotechnology & Applied Microbiology

Engineered pegRNAs improve prime editing efficiency

James W. Nelson et al.

Summary: Prime editing technology enhances editing efficiency by optimizing the structure of pegRNAs, preventing degradation of the 3' region from affecting system activity, without increasing the risk of off-target editing.

NATURE BIOTECHNOLOGY (2022)

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Article Multidisciplinary Sciences

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

J. Ferreira da Silva et al.

Summary: Prime editing is a powerful genome engineering approach that can introduce various modifications into the genome. This study identifies the mismatch repair pathway as inhibitory for Prime editing, and loss of mismatch repair enhances its efficiency.

NATURE COMMUNICATIONS (2022)

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Letter Biochemistry & Molecular Biology

Multiplex precision gene editing by a surrogate prime editor in rice

Huiyuan Li et al.

MOLECULAR PLANT (2022)

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Article Biotechnology & Applied Microbiology

An engineered prime editor with enhanced editing efficiency in plants

Yuan Zong et al.

Summary: In this study, we have improved the editing efficiency of prime editing by engineering the structure of the prime editor and combining it with specific guide RNAs. The optimized prime editor showed significantly increased editing efficiency in plant cells, and when combined with engineered guide RNAs, it resulted in the generation of herbicide-tolerant rice plants.

NATURE BIOTECHNOLOGY (2022)

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Letter Biology

Improving the efficiency of prime editing with epegRNAs and high-temperature treatment in rice

Jinpeng Zou et al.

SCIENCE CHINA-LIFE SCIENCES (2022)

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Letter Biochemistry & Molecular Biology

Optimized prime editing efficiently generates glyphosate-resistant rice plants carrying homozygous TAP-IVS mutation in EPSPS

Yuanyuan Jiang et al.

MOLECULAR PLANT (2022)

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Article Biotechnology & Applied Microbiology

Development of a highly efficient prime editor 2 system in plants

Juan Li et al.

Summary: In this study, an enhanced plant Prime Editor 2 system (enpPE2) is developed to improve editing efficiency. The results demonstrate that enpPE2 is a robust and powerful tool for precise modification of plant genomes.

GENOME BIOLOGY (2022)

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Article Plant Sciences

A design optimized prime editor with expanded scope and capability in plants

Wen Xu et al.

Summary: This study demonstrated that using N-terminal reverse transcriptase-Cas9 nickase fusion and introducing multiple-nucleotide substitutions in the reverse transcriptase template can significantly enhance the efficiency of prime editing in plants and human cells. The optimized approach achieved higher editing frequencies compared to the original editor, Prime Editor 3, showing the potential to make previously non-editable target sites editable and expand the capabilities of prime editing in the future.

NATURE PLANTS (2022)

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Article Biochemistry & Molecular Biology

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Peter J. Chen et al.

Summary: The study found that DNA mismatch repair (MMR) hinders prime editing and promotes undesired byproducts, but the efficiency of substitution, small insertion, and small deletion edits can be enhanced by developing PE4 and PE5 prime editing systems. Strategic silent mutations near the intended edit and optimization of prime editor protein also contribute to improved editing outcomes.

CELL (2021)

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Article Biotechnology & Applied Microbiology

Rice MutLγ, the MLH1-MLH3 heterodimer, participates in the formation of type I crossovers and regulation of embryo sac fertility

Bigang Mao et al.

Summary: The development of embryo sacs in plants is crucial for seed production. A study on a rice female sterile mutant (fsv1) found that the defective gene OsMLH3 plays a key role in crossover formation in mother cells, affecting the meiotic process and seed setting in hybrid rice production.

PLANT BIOTECHNOLOGY JOURNAL (2021)

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Article Biotechnology & Applied Microbiology

High-efficiency prime editing with optimized, paired pegRNAs in plants

Qiupeng Lin et al.

Summary: Designing prime binding sites with a certain melting temperature and using two pegRNAs can substantially enhance the efficiency of prime editing.

NATURE BIOTECHNOLOGY (2021)

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Article Biochemistry & Molecular Biology

OsMLH1 interacts with OsMLH3 to regulate synapsis and interference-sensitive crossover formation during meiosis in rice

Xiaodong Xin et al.

Summary: Meiotic recombination is essential for genetic exchange in sexually reproducing organisms. OsMLH1 is crucial for male fertility and crossover formation in rice, interacting with OsMLH3. The assembly of synapsis complex relies on OsMLH1.

JOURNAL OF GENETICS AND GENOMICS (2021)

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Article Plant Sciences

Identification of herbicide resistance OsACC1 mutations via in planta prime-editing-library screening in rice

Rongfang Xu et al.

Summary: A prime-editing-library-mediated saturation mutagenesis method was developed to expand herbicide-resistant mutations in plants efficiently. This method identified 16 types of herbicide-resistance-endowing mutations at conserved residues of OsACC1, demonstrating its advantage over base-editing-mediated mutagenesis.

NATURE PLANTS (2021)

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Article Multidisciplinary Sciences