Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 65, Issue 4, Pages 900-906Publisher
WILEY
DOI: 10.1111/jipb.13428
Keywords
CRISPR; Cas; epegRNAs; herbicide resistance; maize; MLH1dn; prime editing
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This study tested PEs optimized through three strategies in maize and demonstrated that the ePE5max system efficiently generated heritable mutations that conferred resistance to herbicides in maize target genes.
Low efficiency is the main obstacle to using prime editing in maize (Zea mays). Recently, prime-editing efficiency was greatly improved in mammalian cells and rice (Oryza sativa) plants by engineering prime-editing guide RNAs (pegRNAs), optimizing the prime editor (PE) protein, and manipulating cellular determinants of prime editing. In this study, we tested PEs optimized via these three strategies in maize. We demonstrated that the ePE5max system, composed of PEmax, epegRNAs (pegRNA-evopreQ. 1), nicking single guide RNAs (sgRNAs), and MLH1dn, efficiently generated heritable mutations that conferred resistance to herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetolactate synthase (ALS), or acetyl CoA carboxylase (ACCase) activity. Collectively, we demonstrate that the ePE5max system has sufficient efficiency to generate heritable (homozygous or heterozygous) mutations in maize target genes and that the main obstacle to using PEs in maize has thus been removed.
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