Glucose facilitates the acclimation of organohalide-respiring bacteria

Organohalide respiring bacteria (OHRB) are the mainstay for bioremediation of organohalide contaminated sites. Enrichment screening of OHRB is prerequisite for the development of high performance dehalogenating bacterial agents. Herein, different domestication strategies were formulated for the main factors (nutrients and inocula) affecting the enrichment of OHRB, and the dehalogenation effect was verified with 2-chlorophenol and per/ polyfluoroalkyl substances. The nutrients had a greater impact on the dehalogenation of the systems relative to the inocula, where the combination of glucose and anaerobic sludge (Glu-AS) had a faster degradation rate (26 +/- 2.5 mu mol L-1 d-1) and more complete dechlorination effectiveness. Meanwhile, the dehalogenation results for perfluorooctanoic acid and trifluoroacetic acid showed the biological defluorination was closely related to the position of fluoride. Further, the microbial community structure profiled the resource competition, metabolic cross-feeding and nutrient dynamic exchange among fermenting bacteria, OHRB and methanogenic bacteria under different domestication strategies as endogenous factors affecting the dehalogenation performance, and speculated a hypothetical model for the interaction of different functional bacteria. Our research contributed guidelines and references for the development of efficient dehalogenating bacterial agents, and provided scientific theoretical and technical support for promoting the maximum efficiency of bioremediation of organohalogenated sites.

Automated summary

Organohalide respiring bacteria (OHRB) play a crucial role in the bioremediation of organohalide contaminated sites. Different domestication strategies affect the enrichment of OHRB, with nutrients having a greater impact on dehalogenation than inocula. The combination of glucose and anaerobic sludge (Glu-AS) showed faster degradation and more complete dechlorination efficacy. The microbial community structure provides insights into resource competition and nutrient dynamics, contributing to the development of efficient dehalogenating bacterial agents.