An ultrahigh-performance liquid chromatography-fluorescence detection (UHPLC-FLD) method for simultaneous determination of albendazole and its three metabolites in poultry eggs

Albendazole (ABZ) is widely used in the treatment of animal intestinal parasitic infections. This study has established a method of ultrahigh-performance liquid chromatography coupled with fluorescence detection for the simultaneous determination of ABZ and its three metabolites (albendazole, albendazole sulfone, albendazole sulfoxide, albendazole-2-aminosulfone) in poultry (chicken, duck, and goose) eggs. Good linearity was shown under the optimized conditions with the determination coefficient (>= 0.9994). The limits of detection (LOD) were 0.3-4.6 mu g/kg, whereas the limits of quantification (LOQ) were 1.0-12.9 mu g/kg. The recoveries of ABZ and its three metabolites ranged from 80.5% to 98.6%, while relative standard deviations were in the range of 1.2-4.8%, and all target analytes could be detected within 6 min. The method was demonstrated to be sensitive, selective, and quick for the detection of ABZ and its three metabolites.

Automated summary

A sensitive, selective, and quick method for the simultaneous detection of Albendazole and its three metabolites in poultry eggs has been established. The method showed good linearity and recoveries, and all target analytes could be detected within a short period of time.