Novel Green Fluorescent Probe Stem From Carbon Quantum Dots for Specific Recognition of Tyrosinase in Serum and Living Cells

Tyrosinase (TYR), an important biomarker for melanoma, offered significant information early detection of melanoma and may decrease the likelihood of mortality. Therefore, this article constructed a highly sensitive and selective green fluorescent functionalized carbon quantum dots (TYR-CQDs) for tyrosinase (TYR) activity detection by one-step hydrothermal protocol utilizing catechol, citric acid and urea as precursors. The prepared TYR-CQDs illustrated excellent linear relationship and broad linear range with a low detection limit, which exhibited high accuracy and recovery in quantitative determination of TYR in human serum samples. Furthermore, the TYR-CQDs had successfully realized intracellular TYR detection owing to excellent biocompatibility, high anti-interference ability and good cellular imaging capability, suggesting the potential biomedical applications in early diagnosis of melanoma and other tyrosinase-related diseases.

Automated summary

This study successfully constructed highly sensitive and functional carbon quantum dots for the detection of tyrosinase activity. These carbon quantum dots showed excellent linear relationship and broad linear range with high accuracy and recovery in human serum samples. Furthermore, they demonstrated good cellular imaging capability and biocompatibility, making them suitable for intracellular tyrosinase detection. This research holds potential biomedical applications in early diagnosis of melanoma and other tyrosinase-related diseases.