Univariate and multivariate plasticity in response to incubation temperature in an Australian lizard

Environments, particularly developmental environments, can generate a considerable amount of phenotypic variation through phenotypic plasticity. Plasticity in response to incubation temperature is well characterised in egg-laying reptiles. However, traits do not always vary independently of one another, and studies encompassing a broad range of traits spanning multiple categories are relatively rare but crucial to better understand whole-organism responses to environmental change, particularly if covariation among traits may constrain plasticity. In this study, we investigated multivariate plasticity in response to incubation across three temperatures in the delicate skink, Lampropholis delicata, and whether this was affected by covariation among traits. At approximately 1 month of age, a suite of growth, locomotor performance, thermal physiology and behavioural traits were measured. Plasticity in the multivariate phenotype of delicate skinks was distinct for different incubation temperatures. Cool temperatures drove shifts in growth, locomotor performance and thermal physiology, while hot temperatures primarily caused changes in locomotor performance and behaviour. These differences are likely due to variation in thermal reaction norms, as there was little evidence that covariation among traits or phenotypic integration influenced plasticity, and there was no effect of incubation temperature on the direction or strength of covariation. While there were broad themes in terms of which trait categories were affected by different incubation treatments, traits appeared to be affected independently by developmental temperature. Comparing reaction norms of a greater range of traits and temperatures will enable better insight into these patterns among trait categories, as well as the impacts of environmental change.

Automated summary

Environments, especially developmental environments, can lead to a significant amount of phenotypic variation through phenotypic plasticity. This study investigated the multivariate plasticity in response to incubation temperature in a skink species and examined whether there was any effect of covariation among traits. The results showed that different incubation temperatures had distinct effects on the multivariate phenotype, with cool temperatures primarily influencing growth, locomotor performance, and thermal physiology, and hot temperatures mainly affecting locomotor performance and behavior. Despite some broad patterns in the affected trait categories, traits appeared to be independently affected by developmental temperature, and there was little influence of covariation among traits on plasticity.