Mechanistic evaluation of chromium bioremediation in Acinetobacter junii strain b2w: A proteomic approach

Growing industrialization and unchecked release of industrial waste, including heavy metals have resulted in disastrous effects on environment. Considering the problem of heavy metal pollution, the present research was designed to study the bioremediation of chromium, a highly toxic and prominent heavy metal pollutant by Acinetobacter junii strain b2w isolated from the Mithi river, Mumbai, India. The bacterial isolate could grow without affecting its growth kinetics up to a concentration of 200 ppm of chromium and showed resistance towards 400 ppm of chromium. It was able to bioremediate 83.06% of total chromium and reduces 98.24% of Cr6+ to C3+ at a concentration of 10 ppm of chromium. The bacterial isolate could grow well at a wide pH range from 5 to 9, salinity of up to 3.5% and could also tolerate heavy metals such as Cd, Zn, As, Hg, Pb and Cu. Thus, indicating its possible on-ground applicability for bioremediation of chromium. Acinetobacter junii bioaccumulate chromium without disrupting the cell integrity and biosorption. However, chromium alters the functional groups on bacterial cell surface and led to decrease in sulfate-containing molecules. Further, the protein expression study has revealed that Cr significantly up-regulates proteins broadly classified under envelope stress responses, oxidative stress responses, energy metabolism and quorum sensing and growth regulator. The possible mecha-nisms of Cr detoxification in Acinetobacter junii strain b2w could be reduction, bioaccumulation and efflux along with neutralization of oxidative stress generated by Cr. Thus, based on bacterial bioremediation potential and its molecular response, it can be proposed that the isolated Acinetobacter junii has potential applicability for chro-mium bioremediation.

Automated summary

The present study focuses on the bioremediation of chromium, a highly toxic and prominent heavy metal pollutant, by the bacterial isolate Acinetobacter junii strain b2w. The isolate demonstrated resistance to high concentrations of chromium and was able to effectively remove and reduce chromium in a controlled experimental setting. It also displayed tolerance to other heavy metals and a wide range of pH and salinity conditions. The study suggests that Acinetobacter junii has the potential to be applied for chromium bioremediation based on its bioremediation capabilities and molecular responses.