4.7 Review

CRISPR/Cas9 technology as an innovative approach to enhancing the phytoremediation: Concepts and implications

Journal

JOURNAL OF ENVIRONMENTAL MANAGEMENT
Volume 323, Issue -, Pages -

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jenvman.2022.116296

Keywords

CRISPR; Cas9 technology; Gene editing; Phytoremediation; Stress tolerance

Funding

  1. Natural Science Foundation of Jiangsu
  2. National Natural Science Foundation of China
  3. Jiangsu Planned Projects for Postdoctoral Research Fund
  4. Jiangsu Funding Program for Excellent Postdoctoral Talent
  5. Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
  6. [BK20211321]
  7. [32271587]
  8. [32171509]
  9. [2021K160B]
  10. [2022ZB658]

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Phytoremediation is an active research field that focuses on identifying and characterizing novel super-accumulators with high chelation action. Molecular tools and CRISPR-Cas9 technology are widely employed to enhance metal absorption, translocation, and tolerance mechanisms in plants for detoxification. Future research in adaptive phytoremediation ability and sustainable ecosystem will be triggered by advancements in molecular science.
Phytoremediation is currently an active field of research focusing chiefly on identifying and characterizing novel and high chelation action super-accumulators. In the last few years, molecular tools have been widely exploited to understand better metal absorption, translocation, cation, and tolerance mechanisms in plants. Recently more advanced CRISPR-Cas9 genome engineering technology is also employed to enhance detoxification efficiency. Further, advances in molecular science will trigger the understanding of adaptive phytoremediation ability plant production in current global warming conditions. The enhanced abilities of nucleases for genome modification can improve plant repair capabilities by modifying the genome, thereby achieving a sustainable ecosystem. The purpose of this manuscript focuses on biotechnology's fundamental principles and application to promote climate-resistant metal plants, especially the CRISPR-Cas9 genome editing system for enhancing the phytor-emediation of harmful contamination and pollutants.

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