4.6 Article

Validation of plasma Torque Teno viral load applying a CE-certified PCR for risk stratification of rejection and infection post kidney transplantation

Journal

JOURNAL OF CLINICAL VIROLOGY
Volume 158, Issue -, Pages -

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ELSEVIER
DOI: 10.1016/j.jcv.2022.105348

Keywords

Torque Teno virus; Immunologic monitoring; Kidney transplantation; Rejection; Infection

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This study demonstrates that quantification of TTV load using a commercial PCR can be used to predict the risk of graft rejection and infection post kidney transplantation. The commercial PCR showed a high correlation with the in-house PCR, but there was a slight difference in results. The results showed that for every 1 log10 increase in TTV load, the risk of graft rejection decreased by 25% and the risk of infection increased by 6%. Therefore, quantification of TTV load using a commercial PCR is of significant value for risk stratification of graft rejection and infection in the first year post kidney transplantation.
Background: Torque Teno virus (TTV) is non-pathogenic, highly prevalent and reflects the immune status of its host. TTV plasma load was suggested for risk stratification of graft rejection and infection post kidneytransplantation, for which most studies applied an in-house PCR. Recently, a commercial PCR was CEcertified for clinical use. The present study was designed to assess the performance of TTV load as quantified by the commercial PCR in the prediction of graft rejection and infection. Methods: Patients and events were selected from the prospective TTV-POET trial, including 683 consecutive adult recipients of a kidney-graft transplanted at the Medical University Vienna, 2016-2020. TTV was quantified in plasma drawn in Months 4-12 post-transplant by in-house and commercial PCR and associated with consecutive infections and graft rejections until Month 12 post-transplantation.Results: A total of 342 samples from 314 patients with 85 biopsies (rejection, n = 18) and 79 infectious events were assessed. The two PCRs were highly associated (estimate 0.91, 95%CI 0.89-0.93), with a mean difference of 1.38 log10 copies/mL (95%CI 1.46-1.30). The risk of rejection decreased by 25% with every log10 increase in TTV load as quantified by commercial PCR (RR 0.75, 95%CI 0.67-0.85), and the risk of infection increased by 6% (RR 1.06, 95%CI 1.00-1.12). Conclusion: These data support the value of TTV quantification by commercial PCR for the risk stratification of graft rejection and infection in the first year post kidney-transplantation. The test performance determined within this study may serve to design clinical trials and subsequently, support application in clinical routine.

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