Ecofriendly and enhanced biogenic synthesis of silver nanoparticles using deep eutectic solvent-based green tea extracts

In compliance with green chemistry, metallic nanoparticles can be biogenically synthesized using plant extracts. Herein, three different deep eutectic solvents (DESs) were used instead of water to prepare green tea extracts (GTEs). The resultant DES-based GTEs exerted different beneficial effects on the biosynthesis of silver nano-particles. Compared with the water-based GTE (W-GTE), the DES-based GTEs contained higher levels of cate-chins (up to 235% more) that are natural reducing and capping agents and showed higher synthesis efficiency. In particular, two DESs, which consist of glycerol and betaine with a common urea molecule and are designated as GU and BU, respectively, could produce silver nanoparticles with superior properties to water. Specifically, GU-and BU-GTE-based silver nanoparticles showed uniformly reduced sizes, 39.12 (+/- 5.33) nm and 43.11 (+/- 6.42) nm, respectively and homogenous face-centered cubic crystallinity. DESs added to W-GTEs lead to synthesis of silver nanoparticles with improved dispersion. The DES-GTE-based silver nanoparticles at 60 mu g/mL displayed in vitro anti-cancer activity comparable to doxorubicin at 12.5 mu g/mL. These results suggest that the tested DESs could serve as efficient extraction solvents for phytochemicals as well as effective surface modifiers without posing adverse effects for subsequent applications of the synthesized nanoparticles. The favorable effects of GU and BU were found to partially involve ammonia, which was formed during DES preparation at high tempera-tures (>= 80 degrees C). This study shows that DESs can promote the biogenic synthesis of silver nanoparticles by playing diverse roles depending on the unique properties of the DESs and their constituents.

Automated summary

According to the principles of green chemistry, metallic nanoparticles can be synthesized using plant extracts. In this study, deep eutectic solvents (DESs) were used to prepare green tea extracts (GTEs) instead of water, resulting in higher synthesis efficiency and improved properties of silver nanoparticles. Among the DESs, the mixture of glycerol and betaine produced silver nanoparticles with uniform size and crystallinity. Additionally, adding DESs to GTEs enhanced the dispersion of silver nanoparticles.