Packed modulation loops to reduce band broadening in two-dimensional liquid chromatography

Article Chemistry, Analytical

Resolving Solvent Incompatibility in Two-Dimensional Liquid Chromatography with In-Line Mixing Modulation

Shijia Tang et al.

Summary: This article presents a facile strategy of connecting an in-line mixer, in-line mixing modulation (ILMM), to achieve challenging 2D-LC workflows. ILMM significantly reduces peak distortion and improves the accuracy and sensitivity of the analysis. It also offers a simple and flexible system configuration, making it a potential tool for broader 2D-LC applications.

ANALYTICAL CHEMISTRY (2022)

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Article Biochemical Research Methods

Peak-tracking algorithm for use in comprehensive two-dimensional liquid chromatography-Application to monoclonal-antibody peptides

Stef R. A. Molenaar et al.

Summary: An algorithm was developed for peak-tracking in comprehensive two-dimensional liquid chromatography coupled to mass spectrometry, utilizing spectral information and statistical moments to track chromatographic peaks and assess pairing validity. The algorithm was applied to peptide retention data and complex peptide mixtures, yielding no false positives but some unpaired trace compounds due to limitations in peak-detection.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Article Biochemical Research Methods

Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography

Ali Moussa et al.

Summary: The study aimed to understand and quantify the dispersion occurring in open tubular interface loops using numerical simulation methods and experimental measurements. It was found that for a straight loop capillary, the peak variance depends on a dimensionless parameter and the ratio of filling and emptying flow rates significantly impacts the result. Additionally, when a tightly coiled loop is used instead of a straight capillary, smaller peak variances are observed.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Article Biochemical Research Methods