Journal
JOURNAL OF CELL BIOLOGY
Volume 222, Issue 1, Pages -Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.202202064
Keywords
-
Categories
Funding
- National Key Research and Development Program of China [2017YFA0503600]
- National Natural Science Foundation of China [32070707, 31871350, 31621002]
- Fundamental Research Funds for the Central Universities [WK9110000151]
Ask authors/readers for more resources
This study reveals the crucial role of the Cdc42 GTPase-activating protein (GAP) Rga6 in the monopolar growth of fission yeast spores. The absence of Rga6 leads to an increase in bipolar growth of spores. Rga6 decorates the non-growing cortical region, binds phosphatidylinositol 4,5-bisphosphate, and co-localizes with the phosphatidylinositol 4,5-bisphosphate-binding protein Opy1.
The molecular mechanisms underlying the establishment of the monopolar growth of fission yeast spores have been less characterized. Here, we report that the Cdc42 GTPase-activating protein (GAP) Rga6 is required for promoting monopolar growth during spore germination. The absence of Rga6 increases the number of spores that grow in a bipolar fashion. Rga6 decorates the non-growing cortical region, binds phosphatidylinositol 4,5-bisphosphate, and colocalizes with the phosphatidylinositol 4,5-bisphosphate-binding protein Opy1. Overexpression of Opy1 diminishes the cortical localization of Rga6. The characteristic localization of Rga6 on the cell cortex depends on the C-terminal PBR region of Rga6. Moreover, engineered chimera composed of the Rga6 C-terminal PBR region fused to the GAP domain of Rga3 or Rga4 are sufficient to rescue the spore growth phenotype caused by the absence of Rga6. Hence, our work establishes a paradigm in which the lipid composition of the plasma membrane directs polarized cell growth by specifying the cortical localization of a GAP protein.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available